This mark is automatically generated based on current and recent activity in the IV/LAB.

* The number of lab members who have logged in to our linux machines in the past 24 hours is 1.
* The number of lab members who have logged in to our linux machines this week is 1.
* The current size of the directory for our published research papers is 24331079 KB.
* The lights in the lab are on.

Modeling distributed forces within cell adhesions of varying size on continuous substrates

Journal Article

Modeling distributed forces within cell adhesions of varying size on continuous substrates. Jay C. Hou, Ghaidan A. Shamsan, Sarah M. Anderson, Mariah M. McMahon, Liam P. Tyler, Brian T. Castle, Rachel K. Heussner, Paolo P. Provenzano, Daniel F. Keefe, Victor H. Barocas, David J. Odde. Cytoskeleton (2019) Volume 76, Number 11-12 pp. 571-585

Abstract

Cell migration and traction are essential to many biological phenomena, and one of their key features is sensitivity to substrate stiffness, which biophysical models, such as the motor‐clutch model and the cell migration simulator can predict and explain. However, these models have not accounted for the finite size of adhesions, the spatial distribution of forces within adhesions. Here, we derive an expression that relates varying adhesion radius ( R ) and spatial distribution of force within an adhesion (described by s ) to the effective substrate stiffness ( κ sub), as a function of the Young's modulus of the substrate ( E Y ), which yields the relation, urn:x-wiley:19493584:media:cm21561:cm21561-math-0001, for two‐dimensional cell cultures. Experimentally, we found that a cone‐shaped force distribution ( s = 1.05) can describe the observed displacements of hydrogels deformed by adherent U251 glioma cells. Also, we found that the experimentally observed adhesion radius increases linearly with the cell protrusion force, consistent with the predictions of the motor‐clutch model with spatially distributed clutches. We also found that, theoretically, the influence of one protrusion on another through a continuous elastic environment is negligible. Overall, we conclude cells can potentially control their own interpretation of the mechanics of the environment by controlling adhesion size and spatial distribution of forces within an adhesion.

Projects

This publication is a part of the following research projects:

    Links